THE RADIOACTIVE RADIATION INDUCES DELAYED, MITOCHONDRIA-DEPENDENT OXIDATIVE STRESS IN CELLS OF LEUKEMIA K562

Saenko Yury Vladimirovich, Candidate of biological sciences, senior staff scientist, Center of nanotechnologis and materials, Ulyanovsk State University, saenkoyv@yandex.ru
Shutov Alexander Mikhaylovich, Doctor of medical sciences, professor, head of sub-department of therapeutics and occupational diseases, Ulyanovsk State University, amshu@mail.ru
Napalkova Svetlana Mikhaylovna, Doctor of biological sciences, professor, head of sub-department of general and clinical pharmacology, Ulyanovsk State University, kfarm@ulsu.ru
Rastorgueva Evgeniya Vladimirovna, Senior lecturer, sub-department of general and clinical pharmacology with a course of microbiology, Ulyanovsk State University, kfarm@ulsu.ru
Maslakova Anna Gennadyevna, Postgraduate student, Ulyanovsk State University, kfarm@ulsu.ru
Serova Diana Valeryevna, Student, Ulyanovsk State University, kfarm@ulsu.ru

577.2.043:539.1

The authors have studied the development dynamics and sources of radiation-induced accumulation of reactive oxygen species and the role of mitochondria in this process using cell cultures of leukemia K562. Intracellular concentration of reactive oxygen species (ROS), in presence or without the rotenone and the mitochondrial potential were analyzed after 15, 30 minutes, 1, 4, 8, 12, 24, 48 hours after irra-diation by X-rays at the doses of 4 and 12 Gy. Radiation-induced generation of ROS in K562 cells has two maximum in time, the first peak recorded after 30 minutes and the second - 24 hours after exposure. Adding rotenone to cells at 24 and 48 hours after irradiation reduced the level of ROS. Increased concentrations of ROS accompanied by increased mitochondrial potential. Mitochondria are re-sponsible for the growth of the concentration of ROS in the period 12-48 h after ir-radiation.

oxidative stress, mitochondrial potential, X-ray radiation, reactive oxygen species.